Evaluations of Ovarian and Luteal Blood Flow Waveform Patterns in Buffalos Subjected to OvSynch Protocol in Cold and Hot Seasons

This current study aimed to determine ovarian and luteal blood flow waveform patterns in buffalos synchronized using OvSynch protocol cold hot seasons. Six cyclic buffalo cows aged 6±0.5 years old, having a weight of 400 ± 50 kg, were scanned daily along three successive estrous cycles transrectally by Doppler ultrasonography evaluate the normal hemodynamic during spontaneous ovulation before start experiments. Buffaloes with gonadotropin[GnRH] –prostaglandin[P] –gonadotropin (GPG) which animals received 10μg GnRH on day ??, 0.250μg PGF2α 7, another dose was administered 48h after injection. Blood sampling ultrasound examinations (color spectral modes) conducted phases. Results revealed that peak systolic velocity (PSV) significantly (P<0.05) increased season compared season. The Luteal end days (5,7,9, 11) than one. serum levels estradiol (E2) nitric oxide (NO) second injection elevated as one.
 Moreover, progesterone (P4) had risen OvSynch-treated buffaloes 5,7,9, 11 cycle Conclusion: In season, hemodynamics improved one; this may influence reproductive efficiency buffaloes. Further studies needed prove it.